When you are able, please make 50 culture slants:
1. UV sterilize 50 cryo tubes with lids off (open side up) for 45+ minutes.
2. Prepare 50 mL of quarter strength PDA by dividing normal ingredient amounts by 20.
3. After autoclaving the media (15 min), prop up the cryo tubes (in box) and aliquot 1 mL of media into each tube.
4. Leave the tubes open under the hood to dry and do not UV sterilize.
Thanks! You have done this before and done a great job, so if I left out any details just do what you normally have done.