Thank you in advance for your help with Latasha. Here is a summary of what we will do with Latasha on her first visit:
If she has beetles, we will identify them, and grind them up to extract symbionts. Here is how. We will plate them and wait a week.
If she doesn’t have beetles, or only species that are not easy to tell a story about, we will use our fungi.
– Raffaelea subfusca from Craig
– Ambrosiella hartigii from Martin Sigut
– Fusarium sp. from Martin Sigut
– Raffaelea lauricola from Marc
– Diplodia from oaks as a free-living control (or some better species?)
If these fungi are in decently clean cultures, we can subculture them directly into a competitive assay. If they need some cleanup, we will first subculture them alone.
Competitive assay: We will record single-direction reactions: each field has a record of the reactions of the fungus in the upper row. We also need reactions of each to itself, and a growth rate without a competitor. Can we do two replicates of each assay?
|R sub||R lau||A har||F sp||Dipl|
|R sub||same-growth decrease?||R lau reaction||A har reaction||F sp reaction||Dipl reaction|
|R lau||R sub reaction||same-growth decrease?||A har reaction||etc||etc|
|no competitor||R sub growth||etc|
Better ideas welcome!
How should we score it? How about Strong inhibition, medium, weak, no effect? Or estimated percentage of growth decrease compared to the no-competitor control??