Tanahashi et al. – To obtain the basic artificial diets, agar powder (450 mg) (Nacalai Tesque, Kyoto, Japan), mycelial powder (100 mg), and deionised water containing the preservatives (15 ml) were placed in a 50-ml glass test-tube. Test tubes were autoclaved at 121 °C for 15 min.
Agar with wood only may serve as control.
To obtain mycelia of each fungal species, 5-mm diameter disks of fungal mat were removed from PDA plates, floated singly on 15 ml of aqueous potato dextrose broth (PD broth) (24 g/L) in 50-ml flasks (Sigma Aldrich, St. Louis, MO, USA), and incubated at 25 °C for 10 days. Residues (=mycelia) of fungal suspension by suction filtration were rinsed with deionized water several times and freeze-dried for 24 hours. Dried mycelia were ground with mortar and pestle to 0.5 mm mesh powder and then stored in a desiccator Tanahashi et al., 2009.
Sorbic acid can be used as anti-microbial agent.
- Sorbic acid (0.828 g)
- L(+)-ascorbic acid (1.0 g)
- 133 and sodium hydrocarbonate (1.1 g) in deionized water (1000 ml) (Tanahashi et al., 2009).
streptomycin sulphate and chlortetracycline hydrochloride, at three different contents of 30, 60, and 300 ppm.Tanahashi et al., 2009 Use no more than 5˚C when using antibiotics.
Add ascorbic acid to prevent oxygenation of diets in the autoclave. Can add sodium hydrocarbonate to pre-autoclaved diets to raise the pH to ca 5.5.