We received about 30 beetle samples from a collaborator in Italy, they are in a box in the lab freezer. Some of them are very important, please stop by and I will explain. I would like you to do two things with them:
1) Incorporate them into our database and our -80 freezer: record the samples into the database (see spreadsheet in an email), put new labels in, write our vial numbers on the tubes (side and top), and put them in the freezer.
2) Choose ONE well-preserved specimen of Xyleborus eurygraphus from one of three vials that contain it and extract DNA from it. Use either the Qiagen protocol for tissues or the new OmniPrep (should be slightly better). The extract will then be amplified with three primer pairs that you get from Caroline, for a phylogenetic study that she is doing. Before you do that, I will show you how to record a DNA extraction event and a PCR event into the database.