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Articles posted by adamblack

Rearing Beetles from Naturally Infested Wood

Posted on April 3, 2013 by adamblack in Beetles

Sealing ends of logs against dessication:

  • with parafilm (quickest)
  • paint over with natural latex
  • dip in molten wax (most reliable)

Ideal rearing box
Long, skinny, transparent plastic tupperware with snug lid.
Options for the surface inside:

  • paint with latex and cover with sand immediately, to create rough surface
  • roughen with sand paper (not reliable, droplets still catching beetles)
  • put paper towels on the bottom (keeps moisture well, but not reliable, doesn’t cover corners, which is where beetles congregate)

Utensils for Culturing Fungi

Posted on April 3, 2013 by adamblack in Fungi

List of utensils for fungus culture work:

  • burner
  • ethanol bath for utensils
  • ethanol spray
  • petri dishes for dissection
  • tape
  • forceps
  • gloves
  • Kimwipes
  • lighter
  • media plates
  • parafilm
  • permanent lab marker
  • scalpel
  • scissors
  • pipette and tips for melting & crushing
  • Tween
  • (loop for yeasts)

samples:

  • vials with EtOH and labels for beetle vouchers
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(c) 2013 Ambrosia Symbiosis Lab Protocols

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