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Home» Brian » Culture Slants

Culture Slants

Hi Brian,

When you are able, please make 50 culture slants:

1. UV sterilize 50 cryo tubes with lids off (open side up) for 45+ minutes.
2. Prepare 50 mL of quarter strength PDA by dividing normal ingredient amounts by 20.
3. After autoclaving the media (15 min), prop up the cryo tubes (in box) and aliquot 1 mL of media into each tube.
4. Leave the tubes open under the hood to dry and do not UV sterilize.

Thanks! You have done this before and done a great job, so if I left out any details just do what you normally have done.

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