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Home» Beetles » Extraction from galleries

Extraction from galleries

Materials

  • grinding tool
  • scalpel
  • hood time
  • strong forceps
  • vials
  • ethanol in petri dish
  • vial rack

Procedure

Scrape piece of 1 gallery in the hood, scrape into

a. 1 ml of 1X PBS AUTOCLAVED phosphate buffer saline solution in tube

3. mortar & pestle
4. plate directly, refrigeration decreases growing success

5. Plate three different dilutions, refer to the appropriate protocol.

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