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Home» Bacteria » Field Work

Field Work

Equipment for field sampling

  • autoclaved screw-top vials with moist paper (if sampling live beetles)
  • autoclaved empty screw-top vials
  • ethanol
  • squirt bottle (or separate vials with ethanol)
  • bullet box for vials
  • larger tubes
  • chainsaw + gas + oil
  • 2 broad chisels
  • crow bar
  • anvil pruner
  • folding saw
  • hammer
  • hatchet
  • soft forceps on a string
  • hard forceps
  • scalpel
  • scalpel blades
  • lighter (for forceps & scalpel sterilization)
  • ziploc bags
  • permanent markers (alcohol-resistant)
  • pigma pen for labels
  • notebook

 

In the field

  1. beetles: pierce head, put in numbered vial, record
  2. larvae & frass: put in numbered vial or tube, record
  3. fungi: same as above
  4. frontalis: small samples tied up with rubber band, in ziploc bag

 

Sampling bacteria from beetles

Up to 3 larval chambers, and up to 3 adult beetles (ideally new generation) per tree

1. surface wash 2. the beetle crushed (elytra removed, surface-washed in bleach, water, ethanol, and dried out)

 

Materials for dissection

(all autoclaved)

  • eppendorf vials
  • pestles
  • (chisel)
  • pruner
  • pipette
  • 1000 uL pipette tips
  • EtOH bath
  • scalpel
  • vial rack
  • surface wash for adult beetles (elytra removed, surface-washed in bleach, water, ethanol, and dried out)
  • buffer

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