Ambrosia Symbiosis > Lab Protocols
  • Back to Ambrosia Symbiosis Main
  • Lab
  • Create Protocol
  • Edit Protocols
Home» Fungi » Plate photography

Plate photography

 

  1. Download Canon EOS Utility to your computer: Download link
    (Listed as “EOS Digital Solution Disk Software”)
  2. Ensure photo setup is as pictured (in the flow hood in Adam’s office on the 2nd floor):
    IMG_1341
  3. Hook your computer up to the camera to enable live shooting and direct photo downloads.
  4. Turn on the hood blower and wipe down all surfaces with 70% ethanol.
  5. Set the camera dial to M (Manual) and adjust to the following settings:
    Manual focus
    Large resolution (use Raw for poster photos)
    Shutter 1/100
    F9.0
    ISO 400
    Spot metering
    Flash white balance setting
    WB Shift: B5 G2
    As it appears on the EOS Utility:
    EOS plate settings
  6. Set a plate open-side-up underneath the camera and manually focus the camera to the top of the agar.
  7. Add a piece of paper displaying the underlying plate number in the photo.
  8. Proceed taking photographs of the top and bottom of each plate. Settings do not need to be altered between plates/photographs.
  9. Save photographs to our lab drive (Forest Entomology:\Plate photos) and name them according to plate number (eg: “plate number XXXX.jpg”)
  10. Keep the plate at 4-10° C until ready for discard. Plates should be discarded into an autoclave bag.

    **Note on batteries for flash: Only use Panasonic eneloop batteries. All batteries should be equally charged. Disconnect batteries after use. Battery charger is in camera drawer in Hulcr lab.

View Lab Protocols

  • expandBacteria
  • expandBeetles
  • expandCurrent Work
  • expandDatabase
  • expandFungi
  • expandGeneral Molecular Work
  • expandMiscellaneous
  • expandPathology
  • expandSequence Analysis

(c) 2013 Ambrosia Symbiosis Lab Protocols

Cleantalk Pixel