{"id":1175,"date":"2013-03-14T21:09:34","date_gmt":"2013-03-14T21:09:34","guid":{"rendered":"http:\/\/www.ambrosiasymbiosis.org\/2013\/03\/culture-slants\/"},"modified":"2013-03-14T21:09:34","modified_gmt":"2013-03-14T21:09:34","slug":"culture-slants","status":"publish","type":"post","link":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/culture-slants\/","title":{"rendered":"Culture Slants"},"content":{"rendered":"<p>Hi Brian,<\/p>\n<p>When you are able, please make 50 culture slants:<\/p>\n<p>1. UV sterilize 50 cryo tubes with lids off (open side up) for 45+ minutes.<br \/>\n2. Prepare 50 mL of quarter strength PDA by dividing normal ingredient amounts by 20.<br \/>\n3. After autoclaving the media (15 min), prop up the cryo tubes (in box) and aliquot 1 mL of media into each tube.<br \/>\n4. Leave the tubes open under the hood to dry and do not UV sterilize.<\/p>\n<p>Thanks! You have done this before and done a great job, so if I left out any details just do what you normally have done.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Hi Brian, When you are able, please make 50 culture slants: 1. UV sterilize 50 cryo tubes with lids off (open side up) for 45+ minutes. 2. Prepare 50 mL of quarter strength PDA by dividing normal ingredient amounts by 20. 3. After autoclaving the media (15 min), prop up the cryo tubes (in box) [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"","footnotes":""},"categories":[14],"tags":[],"class_list":["post-1175","post","type-post","status-publish","format-standard","hentry","category-brian"],"_links":{"self":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1175","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/comments?post=1175"}],"version-history":[{"count":0,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1175\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/media?parent=1175"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/categories?post=1175"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/tags?post=1175"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}