{"id":1260,"date":"2013-04-03T13:33:00","date_gmt":"2013-04-03T17:33:00","guid":{"rendered":"http:\/\/www.ambrosiasymbiosis.org\/labprotocols\/extraction-from-galleries-2\/"},"modified":"2015-10-19T08:49:53","modified_gmt":"2015-10-19T12:49:53","slug":"extraction-from-galleries-2","status":"publish","type":"post","link":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/extraction-from-galleries-2\/","title":{"rendered":"Extraction from Galleries"},"content":{"rendered":"<h1 id=\"firstHeading\"><\/h1>\n<div id=\"bodyContent\">\n<h2>Protocol<\/h2>\n<h3>Materials<\/h3>\n<ul>\n<li>grinding tool<\/li>\n<li>scrubber<\/li>\n<li>scalpel<\/li>\n<li>hood time<\/li>\n<li>strong forceps<\/li>\n<li>vials<\/li>\n<li>ethanol in petri dish<\/li>\n<li>vial rack<\/li>\n<\/ul>\n<h3>Procedure<\/h3>\n<p>1. surface wash one log in<\/p>\n<blockquote><p><b>a. 1% sodium hypochloride \u2013 1:10 bleach<\/b><br \/>\n<b>b. water<\/b><\/p><\/blockquote>\n<p>2. scrape piece of 1 gallery in the hood, scrape into<\/p>\n<blockquote><p><b>a. 1 ml of 1X PBS AUTOCLAVED phosphate buffer saline solution in tube<\/b><\/p><\/blockquote>\n<p>3. mortar &amp; pestle<br \/>\n4. plate directly, refrigeration decreases growing success<br \/>\n5. actual plating of Dendroctonus samples, testing three media: chitin + antibiotics, WAY + antibiotics, YMEA + streptomycin (for fungi).<\/p>\n<blockquote><p><b>a. add 0.7 ml of distilled water to the sample<\/b><br \/>\n<b>b. bead beating with larger-diameter beads (710-1180 um size, about 1\/3 of vial volume)<\/b><br \/>\n<b>c. plate 0.3 ml of supernatant on each of the three plate types<\/b><\/p><\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Protocol Materials grinding tool scrubber scalpel hood time strong forceps vials ethanol in petri dish vial rack Procedure 1. surface wash one log in a. 1% sodium hypochloride \u2013 1:10 bleach b. water 2. scrape piece of 1 gallery in the hood, scrape into a. 1 ml of 1X PBS AUTOCLAVED phosphate buffer saline solution [&hellip;]<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"yes","footnotes":""},"categories":[4],"tags":[],"class_list":["post-1260","post","type-post","status-publish","format-standard","hentry","category-bacteria"],"_links":{"self":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1260","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/comments?post=1260"}],"version-history":[{"count":2,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1260\/revisions"}],"predecessor-version":[{"id":1860,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1260\/revisions\/1860"}],"wp:attachment":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/media?parent=1260"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/categories?post=1260"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/tags?post=1260"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}