{"id":1264,"date":"2013-04-03T13:37:24","date_gmt":"2013-04-03T17:37:24","guid":{"rendered":"http:\/\/www.ambrosiasymbiosis.org\/labprotocols\/spore-activation\/"},"modified":"2015-10-19T08:48:57","modified_gmt":"2015-10-19T12:48:57","slug":"spore-activation","status":"publish","type":"post","link":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/spore-activation\/","title":{"rendered":"Spore Activation"},"content":{"rendered":"<h1 id=\"firstHeading\"><\/h1>\n<div id=\"bodyContent\">\n<h2>Protocol<\/h2>\n<p><b>Spore Activation<\/b><br \/>\n(Hayakawa &amp; Nonomura, 1989) used the following procedure to re-activate dormant spores of actinomycetes:<\/p>\n<ol>\n<li>prepare either Yeast extract in PBS (6% w\/v) or SDS in PBS (sodium dodecyl sulfate, (0.05% w\/v), buffered to pH 7 by phosphate buffer.<\/li>\n<li>prepare inoculum in PBS buffer<\/li>\n<li>add 0.5 ml of inoculum in PBS buffer to 4.5 ml of enriched buffers<\/li>\n<li>keep on 40C for 20 min<\/li>\n<li>spread on plates (if making WAY, calculate how much yeast to add).<\/li>\n<\/ol>\n<p>My modification \u2013 dissolved yeast extract and SDS directly in PBS, dilluted dry culture pieces in it. \u2013\u00a0<b>DOESN\u2019T WORK<\/b>. Neither heat shock, nor nutrient shock, nor bleach shock work.<br \/>\nSandye Adams: If re-plating from an old culture, simple streaking may not work. Take a chunk of the colony and put it in between two layers of agar. Or, do liquid culture.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Protocol Spore Activation (Hayakawa &amp; Nonomura, 1989) used the following procedure to re-activate dormant spores of actinomycetes: prepare either Yeast extract in PBS (6% w\/v) or SDS in PBS (sodium dodecyl sulfate, (0.05% w\/v), buffered to pH 7 by phosphate buffer. prepare inoculum in PBS buffer add 0.5 ml of inoculum in PBS buffer to [&hellip;]<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"yes","footnotes":""},"categories":[4],"tags":[],"class_list":["post-1264","post","type-post","status-publish","format-standard","hentry","category-bacteria"],"_links":{"self":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1264","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/comments?post=1264"}],"version-history":[{"count":2,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1264\/revisions"}],"predecessor-version":[{"id":1858,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1264\/revisions\/1858"}],"wp:attachment":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/media?parent=1264"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/categories?post=1264"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/tags?post=1264"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}