{"id":1267,"date":"2013-04-03T13:40:25","date_gmt":"2013-04-03T17:40:25","guid":{"rendered":"http:\/\/www.ambrosiasymbiosis.org\/labprotocols\/testing-for-cellulose-degredation\/"},"modified":"2015-10-19T08:48:46","modified_gmt":"2015-10-19T12:48:46","slug":"testing-for-cellulose-degredation","status":"publish","type":"post","link":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/testing-for-cellulose-degredation\/","title":{"rendered":"Testing For Cellulose Degredation"},"content":{"rendered":"<h2>Protocol<\/h2>\n<p><b>Cellulose Degradation<\/b><br \/>\nIsolate possible cellulose degraders on CMC (5 g\/L &amp; salts, false positives possible) or MCC (4g\/L &amp; salts). Use CMC media for Congo Red test \u2013 grow isolate in the plate center for a week, overlay with Congo Red, wash with water, wash with salts (3M NaCl), check for clearing. When isolating cellulose degrading bacteria from wood, it\u2019s good to add antifungal antibiotics, otherwise Trichoderma takes over.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Protocol Cellulose Degradation Isolate possible cellulose degraders on CMC (5 g\/L &amp; salts, false positives possible) or MCC (4g\/L &amp; salts). Use CMC media for Congo Red test \u2013 grow isolate in the plate center for a week, overlay with Congo Red, wash with water, wash with salts (3M NaCl), check for clearing. When isolating [&hellip;]<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"yes","footnotes":""},"categories":[4],"tags":[],"class_list":["post-1267","post","type-post","status-publish","format-standard","hentry","category-bacteria"],"_links":{"self":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1267","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/comments?post=1267"}],"version-history":[{"count":1,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1267\/revisions"}],"predecessor-version":[{"id":1857,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1267\/revisions\/1857"}],"wp:attachment":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/media?parent=1267"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/categories?post=1267"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/tags?post=1267"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}