{"id":1274,"date":"2013-04-03T13:52:29","date_gmt":"2013-04-03T17:52:29","guid":{"rendered":"http:\/\/www.ambrosiasymbiosis.org\/labprotocols\/calculating-colony-forming-units\/"},"modified":"2017-07-27T12:23:20","modified_gmt":"2017-07-27T16:23:20","slug":"calculating-colony-forming-units","status":"publish","type":"post","link":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/calculating-colony-forming-units\/","title":{"rendered":"Calculating Colony Forming Units"},"content":{"rendered":"<h1 id=\"firstHeading\"><\/h1>\n<div id=\"bodyContent\">\n<p>To estimate the initial number of colony-forming units in a mycangium, use the technique of serial dilution. See Database protocols for Isolations databse for how to accurately input this information into the database.<\/p>\n<h2>Isolation<\/h2>\n<ol>\n<li>Prepare two tubes per each sample, label them &#8220;0.1&#8221; and &#8220;0.01&#8221;, fill each with 500 ul of water or PBS.<\/li>\n<li>Suspend mycangium in the tube &#8220;0.1&#8221; and vortex.<\/li>\n<li>Plate\u00a0<b>50 ul<\/b>\u00a0of the suspension on media. Record the plate as &#8220;0.1 dilution&#8221; in [PLATES].[note] in the Isolations database.<\/li>\n<li>Transfer\u00a0<b>50 ul<\/b>\u00a0of the initial suspension to the second tube (&#8220;0.01&#8221;) and vortex.<\/li>\n<li>Plate\u00a0<b>50 ul<\/b>\u00a0of the second suspension on\u00a0<b>second<\/b>\u00a0media plate, and record that plate as &#8220;0.01 dilution&#8221;.<\/li>\n<li>Plate\u00a0<b>5 ul<\/b>\u00a0of the second suspension on a\u00a0<b>third<\/b>\u00a0plate, and record it as &#8220;0.001 dilution&#8221;.<\/li>\n<\/ol>\n<h2>Calculation of CFU count<\/h2>\n<p>Multiply the number of colonies on the plate by the inverse of the initial dilution factor.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>To estimate the initial number of colony-forming units in a mycangium, use the technique of serial dilution. See Database protocols for Isolations databse for how to accurately input this information into the database. Isolation Prepare two tubes per each sample, label them &#8220;0.1&#8221; and &#8220;0.01&#8221;, fill each with 500 ul of water or PBS. Suspend [&hellip;]<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"yes","footnotes":""},"categories":[5],"tags":[],"class_list":["post-1274","post","type-post","status-publish","format-standard","hentry","category-fungi"],"_links":{"self":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1274","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/comments?post=1274"}],"version-history":[{"count":3,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1274\/revisions"}],"predecessor-version":[{"id":1967,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1274\/revisions\/1967"}],"wp:attachment":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/media?parent=1274"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/categories?post=1274"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/tags?post=1274"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}