{"id":1308,"date":"2013-04-08T17:51:16","date_gmt":"2013-04-08T21:51:16","guid":{"rendered":"http:\/\/www.ambrosiasymbiosis.org\/labprotocols\/lactaphenol-blue-slide-mount\/"},"modified":"2015-10-19T08:46:54","modified_gmt":"2015-10-19T12:46:54","slug":"lactaphenol-blue-slide-mount","status":"publish","type":"post","link":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/lactaphenol-blue-slide-mount\/","title":{"rendered":"Lactaphenol blue slide mount"},"content":{"rendered":"<ol>\n<li>Place a drop of 70-95% ethanol on a microscope slide.<\/li>\n<li>Immerse the fungal material in the drop of alcohol.<\/li>\n<li>Add one or at most two drops of the lactophenol blue stain before the alcohol dries out.<\/li>\n<li>Holding the coverslip between forefinger and thumb, touch one edge of the drop of alcohol\/stain with the coverslip edge, and lower gently to avoid air bubbles.<\/li>\n<li>Ring the edge of the coverslip with nail polish to prevent\u00a0desiccation\u00a0 This way, the mount will last for days to months.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Place a drop of 70-95% ethanol on a microscope slide. Immerse the fungal material in the drop of alcohol. Add one or at most two drops of the lactophenol blue stain before the alcohol dries out. Holding the coverslip between forefinger and thumb, touch one edge of the drop of alcohol\/stain with the coverslip edge, [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"yes","footnotes":""},"categories":[5],"tags":[],"class_list":["post-1308","post","type-post","status-publish","format-standard","hentry","category-fungi"],"_links":{"self":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1308","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/comments?post=1308"}],"version-history":[{"count":1,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1308\/revisions"}],"predecessor-version":[{"id":1842,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1308\/revisions\/1842"}],"wp:attachment":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/media?parent=1308"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/categories?post=1308"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/tags?post=1308"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}