Tanahashi et al.<\/b>\u00a0– To obtain the basic artificial diets, agar powder (450 mg) (Nacalai Tesque, Kyoto, Japan), mycelial powder (100 mg), and deionised water containing the preservatives (15 ml) were placed in a 50-ml glass test-tube. Test tubes were autoclaved at 121 \u00b0C for 15 min.
\nAgar with wood only may serve as control.<\/p>\n
To obtain mycelia of each fungal species, 5-mm diameter disks of fungal mat were removed from PDA plates, floated singly on 15 ml of aqueous potato dextrose broth (PD broth) (24 g\/L) in 50-ml flasks (Sigma Aldrich, St. Louis, MO, USA), and incubated at 25 \u00b0C for 10 days. Residues (=mycelia) of fungal suspension by suction filtration were rinsed with deionized water several times and freeze-dried for 24 hours. Dried mycelia were ground with mortar and pestle to 0.5 mm mesh powder and then stored in a desiccator\u00a0Tanahashi et al., 2009<\/b>. Antibacterial additives:<\/b> Protocol Tanahashi et al.\u00a0– To obtain the basic artificial diets, agar powder (450 mg) (Nacalai Tesque, Kyoto, Japan), mycelial powder (100 mg), and deionised water containing the preservatives (15 ml) were placed in a 50-ml glass test-tube. Test tubes were autoclaved at 121 \u00b0C for 15 min. Agar with wood only may serve as control. […]<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"yes","footnotes":""},"categories":[6],"tags":[],"class_list":["post-1324","post","type-post","status-publish","format-standard","hentry","category-beetles"],"_links":{"self":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1324","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/comments?post=1324"}],"version-history":[{"count":1,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1324\/revisions"}],"predecessor-version":[{"id":1834,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1324\/revisions\/1834"}],"wp:attachment":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/media?parent=1324"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/categories?post=1324"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/tags?post=1324"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nSorbic acid can be used as anti-microbial agent.<\/b><\/p>\n\n
\nstreptomycin sulphate and chlortetracycline hydrochloride, at three different contents of 30, 60, and 300 ppm.Tanahashi et al., 2009<\/b>\u00a0Use no more than 5\u02daC when using antibiotics.
\nIn Autoclave<\/b>
\nAdd ascorbic acid to prevent oxygenation of diets in the autoclave. Can add sodium hydrocarbonate to pre-autoclaved diets to raise the pH to ca 5.5.<\/p>\n","protected":false},"excerpt":{"rendered":"