{"id":1337,"date":"2013-04-10T13:41:13","date_gmt":"2013-04-10T17:41:13","guid":{"rendered":"http:\/\/www.ambrosiasymbiosis.org\/labprotocols\/primers-2\/"},"modified":"2015-10-19T08:40:33","modified_gmt":"2015-10-19T12:40:33","slug":"primers-2","status":"publish","type":"post","link":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/primers-2\/","title":{"rendered":"Primers"},"content":{"rendered":"

Diluting primers<\/h2>\n

Reconstituting lyophilized primers to create 100X stock:
\nResuspend to 100 uM\/L concentration: for 28.5 nM of lyophilized primer, add 285 ul water (10 times more, in uL).<\/p>\n

Dilute to make working solution\u00a0:
\nTo 10uL of the working stock above, add 90 uL of water, to make 10 uM\/L (or 10 pM\/ul) solution.<\/p>\n

Working concentration: Use 1uL of the working solution above in 25uL reaction (10\/25=0.4pM per rxn).<\/p>\n

 <\/p>\n

Primers we use<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
gene<\/th>\nprimer code<\/th>\nseq<\/th>\n<\/th>\n<\/tr>\n
16S<\/td>\n1492R<\/td>\nGGTTACCTTGTTACGACTT<\/td>\n<\/td>\n<\/tr>\n
16S<\/td>\n27F<\/td>\n5′-AGAGTTTGATCCTGGCTCAG<\/td>\n<\/td>\n<\/tr>\n
16S bact rev<\/td>\n338R<\/td>\n5′-TGCTGCCTCCCGTAGGAGT<\/td>\nAmann et al. 1995. Microbiol. Rev. 59, 143\u2013169.<\/td>\n<\/tr>\n
16S bact+archaea for<\/td>\n515F<\/td>\n5′-GTGCCAGCMGCCGCGGTAA<\/td>\nBergmann et al 2011 (Fierer lab)<\/td>\n<\/tr>\n
16S bact+archaea rev<\/td>\n806R<\/td>\n5-GGACTACVSGGGTATCTAAT<\/td>\nBergmann et al 2011 (Fierer lab)<\/td>\n<\/tr>\n
cellobiohydrolase<\/td>\nfungCBH1f<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
cellobiohydrolase<\/td>\nfungCBH1r<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
efTu<\/td>\n52F<\/td>\nLudwig et al<\/td>\n<\/td>\n<\/tr>\n
ITS (end of SSU)<\/td>\nITS1<\/td>\nTCCGTAGGTGAACCTGCGG<\/td>\n<\/td>\n<\/tr>\n
ITS (in 5.8S, for)<\/td>\nITS3<\/td>\nGCATCGATGAAGAACGCAGC<\/td>\n<\/td>\n<\/tr>\n
ITS (in 5.8S, rev)<\/td>\nITS2<\/td>\nGCTGCGTTCTTCATCGATGC<\/td>\n<\/td>\n<\/tr>\n
ITS (rev, start of LSU)<\/td>\nITS4<\/td>\nTCCTCCGCTTATTGATATGC<\/td>\n<\/td>\n<\/tr>\n
ITS fungal (like ITS1, basidio)<\/td>\nITS1-F<\/td>\nCTTGGTCATTTAGAGGAAGTAA<\/td>\n<\/td>\n<\/tr>\n
SSU (18S) general<\/td>\nNS1<\/td>\nGTAGTCATATGCTTGTCTC<\/td>\neukaryotes?<\/td>\n<\/tr>\n
SSU rev<\/td>\nNS2<\/td>\nGGCTGCTGGCACCAGACTTGC<\/td>\n550 in S. cerevisiae; eukaryotes?<\/td>\n<\/tr>\n
SSU (18S) general<\/td>\nNS4<\/td>\nCTTCCGTCAATTCCTTTAAG<\/td>\neukaryotes?<\/td>\n<\/tr>\n
SSU fungal<\/td>\nAU2<\/td>\n5′-TTTCGATGGTAGGATAGDGG<\/td>\nVandenkoornhuyse et al. 2002, Science 295:2051<\/td>\n<\/tr>\n
SSU fungal<\/td>\nAU4<\/td>\n5′-RTCTCACTAAGCCATTC<\/td>\nVandenkoornhuyse et al. 2002, Science 295:2051<\/td>\n<\/tr>\n
SSU fungal<\/td>\nJH-SSU-304<\/td>\n5′-CCCTATCAACTTTCGATG<\/td>\nupstream of AU2, 2bp difference from Curculionidae, can use with NS2 for 240bp conserved product or with JH-SSU-822 for ~500bp of variable product<\/td>\n<\/tr>\n
SSU fungal (rev)<\/td>\nJH-SSU-822<\/td>\n5′-CTAGAAACCAACAAAATAG<\/td>\n2bp upstream of SR4, 4bp difference from Curculionidae<\/td>\n<\/tr>\n
SSU fungal (rev)<\/td>\nJH-SSU-1646<\/td>\n5′-CTAAGCCATTCAATCGGTA<\/td>\n6bp upstream of AU4, 2bp difference from Curculionidae<\/td>\n<\/tr>\n
LSU (28S)<\/td>\nLR0R<\/td>\n5′-ACCCGCTGAACTTAAGC<\/td>\nidentical in insect and fungi<\/td>\n<\/tr>\n
LSU (28S) rev<\/td>\nLR2<\/td>\n5′-TTTTCAAAGTTCTTTTC<\/td>\nWhite et al., from yeast, 1bp difference from filamentous ascos<\/td>\n<\/tr>\n
LSU (28S) rev<\/td>\nLR3<\/td>\n5′-CCGTGTTTCAAGACGGG<\/td>\nidentical for beetles and fungi<\/td>\n<\/tr>\n
LSU (28S)<\/td>\nJH-LSU-91<\/td>\n5′-AGTAACGGCGAGTGAAG<\/td>\nprobably universal for fungi, not for insect, position 91 in S. cerevisiae 28S<\/td>\n<\/tr>\n
LSU (28S) rev<\/td>\nJH-LSU-377<\/td>\n5′-TTTTCAAAGTGCTTTTC<\/td>\nalmost identical to LR2 but with one bp more specific to filamentous ascos, position 377 in S. cerevisiae;\u00a0doesn’t work very well<\/b><\/td>\n<\/tr>\n
LSU (28S) rev<\/td>\nJH-LSU-638<\/td>\n5′-GGTCCGTGTTTCAAGAC<\/td>\nreverse complement to Vilgalis’s LR3R (which is not the same as reverse complement of LR3)<\/td>\n<\/tr>\n
LSU (28S) rev<\/td>\nJH-LSU-643<\/td>\n5′-CTCCTTGGTCCGTGTTTC<\/td>\ntested with Raffaelea spp., inconsistent product<\/td>\n<\/tr>\n
LSU (28S) rev<\/td>\nJH-LSU-830<\/td>\n5′-GTTTCCTCTGGCTTCACCC<\/td>\ntested with Raffaelea spp., inconsistent product<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

R= A G (pur<\/b>ine) D= G A T<\/p>\n

Buying primers<\/h2>\n

ITS1 example<\/i>\u00a0CType: DNA Oligos – modified & non-modified
\nOligo Name: …
\nSynthesis Scale: 0.025 \u00b5mol
\nPurification: Desalt
\nSequence (5′ to 3′): …
\nFormat: Lab-ready
\nNumber of Tubes per Oligo: 1
\nConcentration (\u00b5M): NA<\/p>\n

454 primers – NO LONGER IN USE<\/h2>\n

From IDT: to get reaction-ready concentration, get 1nm in 100uL (concentration: 10)
\nOld FLX chemistry\u00a0Fungi<\/b>.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
454 primer<\/th>\nBar Code<\/th>\nlinker<\/th>\ntemplate primer<\/th>\nprimer name<\/th>\nconcatenated<\/th>\nordered<\/th>\n<\/tr>\n
GCCTTGCCAGCCCGCTCAG<\/td>\nnone<\/td>\nTC<\/td>\nAGTAACGGCGAGTGAAG<\/td>\nJH-LSU-091-454B<\/td>\nGCCTTGCCAGCCCGCTCAGTCAGTAACGGCGAGTGAAG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nAACTCGTCGATG<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-201<\/td>\nGCCTCCCTCGCGCCATCAGAACTCGTCGATGAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nAACTGTGCGTAC<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-202<\/td>\nGCCTCCCTCGCGCCATCAGAACTGTGCGTACAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nAAGAGATGTCGA<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-203<\/td>\nGCCTCCCTCGCGCCATCAGAAGAGATGTCGAAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nAAGCTGCAGTCG<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-204<\/td>\nGCCTCCCTCGCGCCATCAGAAGCTGCAGTCGAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nAATCAGTCTCGT<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-205<\/td>\nGCCTCCCTCGCGCCATCAGAATCAGTCTCGTAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nAATCGTGACTCG<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-206<\/td>\nGCCTCCCTCGCGCCATCAGAATCGTGACTCGAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACACACTATGGC<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-207<\/td>\nGCCTCCCTCGCGCCATCAGACACACTATGGCAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACACATGTCTAC<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-208<\/td>\nGCCTCCCTCGCGCCATCAGACACATGTCTACAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACACGAGCCACA<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-209<\/td>\nGCCTCCCTCGCGCCATCAGACACGAGCCACAAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACACGGTGTCTA<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-210<\/td>\nGCCTCCCTCGCGCCATCAGACACGGTGTCTAAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACACTAGATCCG<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-211<\/td>\nGCCTCCCTCGCGCCATCAGACACTAGATCCGAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACACTGTTCATG<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-212<\/td>\nGCCTCCCTCGCGCCATCAGACACTGTTCATGAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACAGACCACTCA<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-213<\/td>\nGCCTCCCTCGCGCCATCAGACAGACCACTCAAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACAGAGTCGGCT<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-214<\/td>\nGCCTCCCTCGCGCCATCAGACAGAGTCGGCTAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACAGCAGTGGTC<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-215<\/td>\nGCCTCCCTCGCGCCATCAGACAGCAGTGGTCAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACAGCTAGCTTG<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-216<\/td>\nGCCTCCCTCGCGCCATCAGACAGCTAGCTTGAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACAGTGCTTCAT<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-217<\/td>\nGCCTCCCTCGCGCCATCAGACAGTGCTTCATAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACAGTTGCGCGA<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-218<\/td>\nGCCTCCCTCGCGCCATCAGACAGTTGCGCGAAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACATCACTTAGC<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-219<\/td>\nGCCTCCCTCGCGCCATCAGACATCACTTAGCAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACATGATCGTTC<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-220<\/td>\nGCCTCCCTCGCGCCATCAGACATGATCGTTCAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACATGTCACGTG<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-221<\/td>\nGCCTCCCTCGCGCCATCAGACATGTCACGTGAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACATTCAGCGCA<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-222<\/td>\nGCCTCCCTCGCGCCATCAGACATTCAGCGCAAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACCACATACATC<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-223<\/td>\nGCCTCCCTCGCGCCATCAGACCACATACATCAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACCAGACGATGC<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-224<\/td>\nGCCTCCCTCGCGCCATCAGACCAGACGATGCAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACCAGCGACTAG<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-225<\/td>\nGCCTCCCTCGCGCCATCAGACCAGCGACTAGAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACCGCAGAGTCA<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-226<\/td>\nGCCTCCCTCGCGCCATCAGACCGCAGAGTCAAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACCTCGATCAGA<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-227<\/td>\nGCCTCCCTCGCGCCATCAGACCTCGATCAGAAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n
GCCTCCCTCGCGCCATCAG<\/td>\nACCTGTCTCTCT<\/td>\nAG<\/td>\nCCGTGTTTCAAGACGGG<\/td>\nLR3-454A-228<\/td>\nGCCTCCCTCGCGCCATCAGACCTGTCTCTCTAGCCGTGTTTCAAGACGGG<\/td>\n20-Aug-2011<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Old FLX chemistry\u00a0bacteria and archaea<\/b>.<\/p>\n\n\n\n\n\n
454 primer<\/th>\nBar Code<\/th>\nlinker<\/th>\ntemplate primer<\/th>\nprimer name<\/th>\n<\/th>\n<\/tr>\n
FLA B primer: GCCTTGCCAGCCCGCTCAG<\/td>\nnone<\/td>\nGA<\/td>\nGTGCCAGCMGCCGCGGTAA<\/td>\n806R-454B-2012Feb<\/td>\n<\/td>\n<\/tr>\n
FLX A primer: GCCTCCCTCGCGCCATCAG<\/td>\nvarious<\/td>\nCC<\/td>\nGGACTACVSGGGTATCTAAT<\/td>\n515f-454A-2012Feb-well_position<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Barcodes on 515R primer ordered on Feb 06 2012 (order “515F-806R-454barcoded”). R primer mixed with each barcoded F primer.<\/p>\n

A1 GTAGTGTCTAGC
\nA2 GTCGCTGTCTTC
\nA3 GTGAGGTCGCTA
\nA4 GTTGACGACAGC
\nA5 GTCACGACTATT
\nA6 GTCTGACAGTTG
\nA7 GTGTGCTATCAG
\nA8 TACAGATGGCTC
\nA9 TACTTACTGCAG
\nA10 GGATCGCAGATC
\nA11 GTAGACTGCGTG
\nA12 GTCAACGCGATG
\nB1 GTCTCTCTACGC
\nB2 GTGTCTACATTG
\nB3 GTATGTTGCTCA
\nB4 GTCTCATGTAGG
\nB5 GTGTACCTATCA
\nB6 TAGCTGAGTCCA
\nB7 GGCGACATGTAC
\nB8 GTAGATGCTTCG
\nB9 GGTGCGTGTATG
\nB10 GCTTACATCGAG
\nB11 GTATCCATGCGA
\nB12 TAACTCTGATGC
\nC1 TACTAATCTGCG
\nC2 TAGCCTCTCTGC
\nC3 GCTTGCGAGACA
\nC4 GTCATATCGTAC
\nC5 GTACGGCATACG
\nC6 GTATGCGCTGTA
\nC7 GTCTATCGGAGT
\nC8 GTGGCGATACAC
\nC9 TACACGATCTAC
\nC10 TACTGGACGCGA
\nC11 TAGCTCGTAACT
\nC12 GGCAGTGTATCG
\nD1 GTAGAGCTGTTC
\nD2 GCTTCATAGTGT
\nD3 TAACAGTCGCTG
\nD4 TACGTGTACGTG
\nD5 TAGCATCGTGGT
\nD6 GTACAAGAGTGA
\nD7 TACGCGCTGAGA
\nD8 TAGATCCTCGAT
\nD9 GCTGTGTAGGAC
\nD10 GGTCGTAGCGTA
\nD11 GTCTGGATAGCG
\nD12 GTGTGTGTCAGG
\nE1 TACAGTCTCATG
\nE2 TACTTCGCTCGC
\nE3 TAGGTATCTCAC
\nE4 GTCGTAGCCAGA
\nE5 GCTGTAGTATGC
\nE6 GGTCACTGACAG
\nE7 GTGATAGTGCCG
\nE8 GTATATCCGCAG
\nE9 GTCATTCACGAG
\nE10 GTCTTCGTCGCT
\nE11 GTGTTGCAGCAT
\nE12 TACATCACCACA
\nF1 TAGACTGTACTC
\nF2 GTAGCGCGAGTT
\nF3 GTCCATAGCTAG
\nF4 TAGTCGTCTAGT
\nF5 GGCTATGACATC
\nF6 GTGACCTGATGT
\nF7 TAGTGCTGCGTA
\nF8 GGTATACGCAGC
\nF9 GTAGCTGACGCA
\nF10 GTCGACTCCTCT
\nF11 GTGACTGCGGAT
\nF12 GTTCGCGTATAG
\nG1 TACGATGACCAC
\nG2 TAGATAGCAGGA
\nG3 TAGTGTGCTTCA
\nG4 GTTAGAGCACTC
\nG5 TACCGCTAGTAG
\nG6 TAGAGAGAGTGG
\nG7 TAAGCGCAGCAC
\nG8 TACTACATGGTC
\nG9 TAGCGACATCTG
\nG10 GGACGTCACAGT
\nG11 GTACTCTAGACT
\nG12 TACACACATGGC
\nH1 TACTGCGACAGT
\nH2 TAGCGGATCACG
\nH3 GTATGACTGGCT
\nH4 GTCTACACACAT
\nH5 GTGCACATTATC
\nH6 GGCGTACTGATG
\nH7 GTAGCAACGTCT
\nH8 TAGCACACCTAT
\nH9 TACGGTATGTCT
\nH10 GTTGTATACTCG
\nH11 GTCGTGTGTCAA
\nH12 GTGCAATCGACG<\/p>\n

The complete order, including the barcodes above:<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
well<\/th>\noligo1<\/th>\noligo2_to_the_same_well<\/th>\n<\/tr>\n
A1<\/td>\nGCCTCCCTCGCGCCATCAGGTAGTGTCTAGCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
A2<\/td>\nGCCTCCCTCGCGCCATCAGGTCGCTGTCTTCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
A3<\/td>\nGCCTCCCTCGCGCCATCAGGTGAGGTCGCTACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
A4<\/td>\nGCCTCCCTCGCGCCATCAGGTTGACGACAGCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
A5<\/td>\nGCCTCCCTCGCGCCATCAGGTCACGACTATTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
A6<\/td>\nGCCTCCCTCGCGCCATCAGGTCTGACAGTTGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
A7<\/td>\nGCCTCCCTCGCGCCATCAGGTGTGCTATCAGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
A8<\/td>\nGCCTCCCTCGCGCCATCAGTACAGATGGCTCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
A9<\/td>\nGCCTCCCTCGCGCCATCAGTACTTACTGCAGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
A10<\/td>\nGCCTCCCTCGCGCCATCAGGGATCGCAGATCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
A11<\/td>\nGCCTCCCTCGCGCCATCAGGTAGACTGCGTGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
A12<\/td>\nGCCTCCCTCGCGCCATCAGGTCAACGCGATGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
B1<\/td>\nGCCTCCCTCGCGCCATCAGGTCTCTCTACGCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
B2<\/td>\nGCCTCCCTCGCGCCATCAGGTGTCTACATTGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
B3<\/td>\nGCCTCCCTCGCGCCATCAGGTATGTTGCTCACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
B4<\/td>\nGCCTCCCTCGCGCCATCAGGTCTCATGTAGGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
B5<\/td>\nGCCTCCCTCGCGCCATCAGGTGTACCTATCACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
B6<\/td>\nGCCTCCCTCGCGCCATCAGTAGCTGAGTCCACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
B7<\/td>\nGCCTCCCTCGCGCCATCAGGGCGACATGTACCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
B8<\/td>\nGCCTCCCTCGCGCCATCAGGTAGATGCTTCGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
B9<\/td>\nGCCTCCCTCGCGCCATCAGGGTGCGTGTATGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
B10<\/td>\nGCCTCCCTCGCGCCATCAGGCTTACATCGAGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
B11<\/td>\nGCCTCCCTCGCGCCATCAGGTATCCATGCGACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
B12<\/td>\nGCCTCCCTCGCGCCATCAGTAACTCTGATGCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
C1<\/td>\nGCCTCCCTCGCGCCATCAGTACTAATCTGCGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
C2<\/td>\nGCCTCCCTCGCGCCATCAGTAGCCTCTCTGCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
C3<\/td>\nGCCTCCCTCGCGCCATCAGGCTTGCGAGACACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
C4<\/td>\nGCCTCCCTCGCGCCATCAGGTCATATCGTACCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
C5<\/td>\nGCCTCCCTCGCGCCATCAGGTACGGCATACGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
C6<\/td>\nGCCTCCCTCGCGCCATCAGGTATGCGCTGTACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
C7<\/td>\nGCCTCCCTCGCGCCATCAGGTCTATCGGAGTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
C8<\/td>\nGCCTCCCTCGCGCCATCAGGTGGCGATACACCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
C9<\/td>\nGCCTCCCTCGCGCCATCAGTACACGATCTACCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
C10<\/td>\nGCCTCCCTCGCGCCATCAGTACTGGACGCGACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
C11<\/td>\nGCCTCCCTCGCGCCATCAGTAGCTCGTAACTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
C12<\/td>\nGCCTCCCTCGCGCCATCAGGGCAGTGTATCGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
D1<\/td>\nGCCTCCCTCGCGCCATCAGGTAGAGCTGTTCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
D2<\/td>\nGCCTCCCTCGCGCCATCAGGCTTCATAGTGTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
D3<\/td>\nGCCTCCCTCGCGCCATCAGTAACAGTCGCTGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
D4<\/td>\nGCCTCCCTCGCGCCATCAGTACGTGTACGTGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
D5<\/td>\nGCCTCCCTCGCGCCATCAGTAGCATCGTGGTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
D6<\/td>\nGCCTCCCTCGCGCCATCAGGTACAAGAGTGACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
D7<\/td>\nGCCTCCCTCGCGCCATCAGTACGCGCTGAGACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
D8<\/td>\nGCCTCCCTCGCGCCATCAGTAGATCCTCGATCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
D9<\/td>\nGCCTCCCTCGCGCCATCAGGCTGTGTAGGACCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
D10<\/td>\nGCCTCCCTCGCGCCATCAGGGTCGTAGCGTACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
D11<\/td>\nGCCTCCCTCGCGCCATCAGGTCTGGATAGCGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
D12<\/td>\nGCCTCCCTCGCGCCATCAGGTGTGTGTCAGGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
E1<\/td>\nGCCTCCCTCGCGCCATCAGTACAGTCTCATGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
E2<\/td>\nGCCTCCCTCGCGCCATCAGTACTTCGCTCGCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
E3<\/td>\nGCCTCCCTCGCGCCATCAGTAGGTATCTCACCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
E4<\/td>\nGCCTCCCTCGCGCCATCAGGTCGTAGCCAGACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
E5<\/td>\nGCCTCCCTCGCGCCATCAGGCTGTAGTATGCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
E6<\/td>\nGCCTCCCTCGCGCCATCAGGGTCACTGACAGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
E7<\/td>\nGCCTCCCTCGCGCCATCAGGTGATAGTGCCGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
E8<\/td>\nGCCTCCCTCGCGCCATCAGGTATATCCGCAGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
E9<\/td>\nGCCTCCCTCGCGCCATCAGGTCATTCACGAGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
E10<\/td>\nGCCTCCCTCGCGCCATCAGGTCTTCGTCGCTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
E11<\/td>\nGCCTCCCTCGCGCCATCAGGTGTTGCAGCATCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
E12<\/td>\nGCCTCCCTCGCGCCATCAGTACATCACCACACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
F1<\/td>\nGCCTCCCTCGCGCCATCAGTAGACTGTACTCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
F2<\/td>\nGCCTCCCTCGCGCCATCAGGTAGCGCGAGTTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
F3<\/td>\nGCCTCCCTCGCGCCATCAGGTCCATAGCTAGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
F4<\/td>\nGCCTCCCTCGCGCCATCAGTAGTCGTCTAGTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
F5<\/td>\nGCCTCCCTCGCGCCATCAGGGCTATGACATCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
F6<\/td>\nGCCTCCCTCGCGCCATCAGGTGACCTGATGTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
F7<\/td>\nGCCTCCCTCGCGCCATCAGTAGTGCTGCGTACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
F8<\/td>\nGCCTCCCTCGCGCCATCAGGGTATACGCAGCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
F9<\/td>\nGCCTCCCTCGCGCCATCAGGTAGCTGACGCACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
F10<\/td>\nGCCTCCCTCGCGCCATCAGGTCGACTCCTCTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
F11<\/td>\nGCCTCCCTCGCGCCATCAGGTGACTGCGGATCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
F12<\/td>\nGCCTCCCTCGCGCCATCAGGTTCGCGTATAGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
G1<\/td>\nGCCTCCCTCGCGCCATCAGTACGATGACCACCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
G2<\/td>\nGCCTCCCTCGCGCCATCAGTAGATAGCAGGACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
G3<\/td>\nGCCTCCCTCGCGCCATCAGTAGTGTGCTTCACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
G4<\/td>\nGCCTCCCTCGCGCCATCAGGTTAGAGCACTCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
G5<\/td>\nGCCTCCCTCGCGCCATCAGTACCGCTAGTAGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
G6<\/td>\nGCCTCCCTCGCGCCATCAGTAGAGAGAGTGGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
G7<\/td>\nGCCTCCCTCGCGCCATCAGTAAGCGCAGCACCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
G8<\/td>\nGCCTCCCTCGCGCCATCAGTACTACATGGTCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
G9<\/td>\nGCCTCCCTCGCGCCATCAGTAGCGACATCTGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
G10<\/td>\nGCCTCCCTCGCGCCATCAGGGACGTCACAGTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
G11<\/td>\nGCCTCCCTCGCGCCATCAGGTACTCTAGACTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
G12<\/td>\nGCCTCCCTCGCGCCATCAGTACACACATGGCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
H1<\/td>\nGCCTCCCTCGCGCCATCAGTACTGCGACAGTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
H2<\/td>\nGCCTCCCTCGCGCCATCAGTAGCGGATCACGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
H3<\/td>\nGCCTCCCTCGCGCCATCAGGTATGACTGGCTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
H4<\/td>\nGCCTCCCTCGCGCCATCAGGTCTACACACATCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
H5<\/td>\nGCCTCCCTCGCGCCATCAGGTGCACATTATCCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
H6<\/td>\nGCCTCCCTCGCGCCATCAGGGCGTACTGATGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
H7<\/td>\nGCCTCCCTCGCGCCATCAGGTAGCAACGTCTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
H8<\/td>\nGCCTCCCTCGCGCCATCAGTAGCACACCTATCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
H9<\/td>\nGCCTCCCTCGCGCCATCAGTACGGTATGTCTCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
H10<\/td>\nGCCTCCCTCGCGCCATCAGGTTGTATACTCGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
H11<\/td>\nGCCTCCCTCGCGCCATCAGGTCGTGTGTCAACCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n
H12<\/td>\nGCCTCCCTCGCGCCATCAGGTGCAATCGACGCCGGACTACVSGGGTATCTAAT<\/td>\nGCCTTGCCAGCCCGCTCAGGAGTGCCAGCMGCCGCGGTAA<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Titanium Lib-a chemistry, NOT YET USED, need to confirm correctness.\u00a0Fungal LSU primers<\/b>. Four-bp multiplex identifiers (MIDs). Only one-directional sequencing needed, from the more variable 3′ end, so only B adapters have multiple MIDs attached.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
adaptor<\/th>\nadaptor seq<\/th>\nkey<\/th>\nMID name<\/th>\nmid<\/th>\nour primer 5-3′<\/th>\n<\/th>\ndirection<\/th>\nname<\/th>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL091<\/td>\nCGTACAGATAT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-091<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL092<\/td>\nCGTAGCTCTCT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-092<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL093<\/td>\nCGTATAGTGCT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-093<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL094<\/td>\nCGTCAGCGACT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-094<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL095<\/td>\nCGTCGCAGTGT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-095<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL096<\/td>\nCGTCTCACGAT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-096<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL097<\/td>\nCGTGACTCAGT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-097<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL098<\/td>\nCTACACGCTCT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-098<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL099<\/td>\nCTACGATATGT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-099<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL100<\/td>\nCTAGACAGACT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-100<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL101<\/td>\nCTAGTACTCAT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-101<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL102<\/td>\nCTATATGTCGT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-102<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL103<\/td>\nCTATCGACACT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-103<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL104<\/td>\nCTATGTAGAGT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-104<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL105<\/td>\nCTCACGTACAT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-105<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL106<\/td>\nCTCGAGTCTCT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-106<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL107<\/td>\nCTCGTCGAGAT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-107<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL108<\/td>\nCTCTACAGCGT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-108<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL109<\/td>\nCTGTCGTGCGT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-109<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL110<\/td>\nCTGTGACGTGT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-110<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL111<\/td>\nGACGCTGTCGT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-111<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL112<\/td>\nGACGTATGACT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-112<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL113<\/td>\nGACTAGCTAGT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-113<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL114<\/td>\nGAGACGTCGCT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-114<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL115<\/td>\nGAGAGAGACGT<\/td>\nCCGTGTTTCAAGACGGG<\/td>\n<\/td>\nrev<\/td>\nLR3-454B-115<\/td>\n<\/tr>\n
A<\/td>\nCGTATCGCCTCCCTCGCGCCA<\/td>\nTCAG<\/td>\nno mid<\/td>\nno mid<\/td>\nAGTAACGGCGAGTGAAG<\/td>\n<\/td>\nfor<\/td>\nJH-LSU-091-454A<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL091<\/td>\nCGTACAGATAT<\/td>\nGTAGTCATATGCTTGTCTC<\/td>\n<\/td>\nrev<\/td>\nNS1-454B-RL091<\/td>\n<\/tr>\n
A<\/td>\nCGTATCGCCTCCCTCGCGCCA<\/td>\nTCAG<\/td>\nno mid<\/td>\nno mid<\/td>\nGGCTGCTGGCACCAGACTTGC<\/td>\n<\/td>\nfor<\/td>\nNS2-454A<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Again – Titanium Lib-A chemistry, not yet tested,\u00a0Bacterial primers<\/b>.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
adaptor<\/th>\nadaptor seq<\/th>\nkey<\/th>\nMID name<\/th>\nmid<\/th>\nour primer 5-3′<\/th>\n<\/th>\ndirection<\/th>\nname<\/th>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL001<\/td>\nAGTCGTGGTGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-001<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL002<\/td>\nATACTAGGTGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-002<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL003<\/td>\nACGAGTGGTGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-003<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL004<\/td>\nATACGTGGCGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-004<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL005<\/td>\nAGTCTACGCGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-005<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL006<\/td>\nACTAGAGGCGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-006<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL007<\/td>\nAGTGTGTGCGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-007<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL008<\/td>\nACACAGTGCGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-008<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL009<\/td>\nACGATCTGCGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-009<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL010<\/td>\nAGAGACGGAGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-010<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL011<\/td>\nACTCGTAGAGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-011<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL012<\/td>\nACGACGGGAGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-012<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL013<\/td>\nACGTCGGGTCT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-013<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL014<\/td>\nACTCTCGGACT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-014<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL015<\/td>\nATAGTAGGACT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-015<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL016<\/td>\nAGACGTCGACT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-016<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL017<\/td>\nAGTGTAGGACT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-017<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL018<\/td>\nACTACTAGACT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-018<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL019<\/td>\nACGTATAGTAT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-019<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL020<\/td>\nAGTACGTGCTG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-020<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL021<\/td>\nACGCGTGGTCG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-021<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL022<\/td>\nAGTACTGGTCG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-022<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL023<\/td>\nACGTAGTGTCG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-023<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL024<\/td>\nATCGACGGACG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-024<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL025<\/td>\nACTACGGGTAG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-025<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL026<\/td>\nACGTACGGTAC<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-026<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL027<\/td>\nATACGTAGGAC<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-027<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL028<\/td>\nACGTCGTGCAC<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-028<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL029<\/td>\nACTCACGGTGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-029<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL030<\/td>\nAGTATGGGTGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-030<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL031<\/td>\nACGCTGTGTGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-031<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL032<\/td>\nATATAGTGTGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-032<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL033<\/td>\nAGAGTCTGTGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-033<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL034<\/td>\nACACGAGGTGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-034<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL035<\/td>\nATCGACAGTGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-035<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL036<\/td>\nAGCGCGCGCGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-036<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL037<\/td>\nACACTCAGCGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-037<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL038<\/td>\nATCTCTGGCGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-038<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL039<\/td>\nAGAGAGGGCGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-039<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL040<\/td>\nATCAGCGGCGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-040<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL041<\/td>\nATGCTAGGCGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-041<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL042<\/td>\nATATCACGAGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-042<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL043<\/td>\nACAGTGGGAGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-043<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL044<\/td>\nAGCTAGGGAGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-044<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL045<\/td>\nATATATGGAGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-045<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL046<\/td>\nACGAGATGAGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-046<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL047<\/td>\nAGTACAGGAGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-047<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL048<\/td>\nAGCGCTAGAGT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-048<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL049<\/td>\nAGTGAGGGTCT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-049<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL050<\/td>\nACTATAGGTCT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-050<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL051<\/td>\nAGATCAGGTCT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-051<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL052<\/td>\nATCTGTAGTCT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-052<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL053<\/td>\nAGAGATAGTCT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-053<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL054<\/td>\nACAGCGTGTCT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-054<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL055<\/td>\nAGACTAGGTCT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-055<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL056<\/td>\nACTACGCGGCT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-056<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL057<\/td>\nACTCGTGGGCT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-057<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL058<\/td>\nACGCACAGGCT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-058<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL059<\/td>\nAGTATCTGGCT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-059<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL060<\/td>\nAGTCGACGGCT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-060<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL061<\/td>\nACGTGCAGACT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-061<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL062<\/td>\nATAGCGGGACT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-062<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL063<\/td>\nACAGACGGACT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-063<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL064<\/td>\nATCGTGGGTAT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-064<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL065<\/td>\nAGCACGGGTAT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-065<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL066<\/td>\nAGTGCTGGTAT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-066<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL067<\/td>\nACTAGCTGTAT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-067<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL068<\/td>\nATACTCTGTAT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-068<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL069<\/td>\nACGTGAGGGAT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-069<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL070<\/td>\nAGACTGAGGAT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-070<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL071<\/td>\nATACGAGGGAT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-071<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL072<\/td>\nACGTCTCGGAT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-072<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL073<\/td>\nAGACGTGGCAT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-073<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL074<\/td>\nAGTAGTCGCAT<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-074<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL075<\/td>\nACTGTCTGGTG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-075<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL076<\/td>\nAGACTCGGGTG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-076<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL077<\/td>\nATCGTAGGGTG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-077<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL078<\/td>\nATATACAGGTG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-078<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL079<\/td>\nACATCGTGGTG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-079<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL080<\/td>\nAGAGTATGGTG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-080<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL081<\/td>\nACAGTAGGCTG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-081<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL082<\/td>\nACTAGAGGCTG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-082<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL083<\/td>\nACGCGACGATG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-083<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL084<\/td>\nATAGTGTGTCG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-084<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL085<\/td>\nACACACTGTCG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-085<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL086<\/td>\nACGATGAGTCG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-086<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL087<\/td>\nATATACGGTCG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-087<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL088<\/td>\nAGCGTACGGCG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-088<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL089<\/td>\nATACGATGGCG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-089<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL090<\/td>\nAGTATAGGGCG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-090<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL091<\/td>\nATATCTGGACG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-091<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL092<\/td>\nAGAGAGCGACG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-092<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL093<\/td>\nAGCACTAGACG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-093<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL094<\/td>\nAGTCGCGGACG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-094<\/td>\n<\/tr>\n
B<\/td>\nCTATGCGCCTTGCCAGCCCGC<\/td>\nTCAG<\/td>\nRL095<\/td>\nACACTGGGACG<\/td>\nTGCTGCCTCCCGTAGGAGT<\/td>\n<\/td>\nrev<\/td>\n338r-454B-095<\/td>\n<\/tr>\n
B<\/td>\nCGTATCGCCTCCCTCGCGCCA<\/td>\nTCAG<\/td>\nno mid<\/td>\nno mid, same for all<\/td>\nAGAGTTTGATCCTGGCTCAG<\/td>\n<\/td>\nfor<\/td>\n27f-454A<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Diluting primers Reconstituting lyophilized primers to create 100X stock: Resuspend to 100 uM\/L concentration: for 28.5 nM of lyophilized primer, add 285 ul water (10 times more, in uL). Dilute to make working solution\u00a0: To 10uL of the working stock above, add 90 uL of water, to make 10 uM\/L (or 10 pM\/ul) solution. Working […]<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"yes","footnotes":""},"categories":[3],"tags":[],"class_list":["post-1337","post","type-post","status-publish","format-standard","hentry","category-general-molecular-work"],"_links":{"self":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1337","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/comments?post=1337"}],"version-history":[{"count":4,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1337\/revisions"}],"predecessor-version":[{"id":1828,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1337\/revisions\/1828"}],"wp:attachment":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/media?parent=1337"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/categories?post=1337"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/tags?post=1337"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}