{"id":1361,"date":"2013-04-16T13:57:53","date_gmt":"2013-04-16T17:57:53","guid":{"rendered":"http:\/\/www.ambrosiasymbiosis.org\/labprotocols\/making-1x-tae\/"},"modified":"2013-04-16T13:57:53","modified_gmt":"2013-04-16T17:57:53","slug":"making-1x-tae","status":"publish","type":"post","link":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/making-1x-tae\/","title":{"rendered":"Making 1X TAE"},"content":{"rendered":"<p>Also could you make 6Liters of 1x TAE to refill the 2 Gallon plastic carboy sitting on the left corner of the sink please?<\/p>\n<p>To make:<\/p>\n<p>Measure out 6L of DI water (from other sink tap, let tap run a few seconds first) into the carboy 1L at a time using the 1L graduated cylinder.<\/p>\n<p>Then measure out 120 mL of 50X TAE buffer (on the shelf with chemicals) using a 100mL graduated cylinder and add to carboy.<\/p>\n<p>Mix the contents of the carboy as best as you can via swirling<\/p>\n<p>Thanks!<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Also could you make 6Liters of 1x TAE to refill the 2 Gallon plastic carboy sitting on the left corner of the sink please? To make: Measure out 6L of DI water (from other sink tap, let tap run a few seconds first) into the carboy 1L at a time using the 1L graduated cylinder. [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"","footnotes":""},"categories":[14],"tags":[],"class_list":["post-1361","post","type-post","status-publish","format-standard","hentry","category-brian"],"_links":{"self":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1361","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/comments?post=1361"}],"version-history":[{"count":0,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1361\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/media?parent=1361"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/categories?post=1361"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/tags?post=1361"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}