{"id":1375,"date":"2013-04-23T13:04:49","date_gmt":"2013-04-23T17:04:49","guid":{"rendered":"http:\/\/www.ambrosiasymbiosis.org\/labprotocols\/latasha-april-25\/"},"modified":"2013-04-23T13:36:09","modified_gmt":"2013-04-23T17:36:09","slug":"latasha-april-25","status":"publish","type":"post","link":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/latasha-april-25\/","title":{"rendered":"Latasha, April 25"},"content":{"rendered":"

Adam,
\nThank you in advance for your help with Latasha. Here is a summary of what we will do with Latasha on her first visit:<\/p>\n

If she has beetles, we will identify them, and grind them up to extract symbionts. Here is how<\/a>. We will plate them and wait a week.<\/p>\n

If she doesn’t have beetles, or only species that are not easy to tell a story about, we will use our fungi.
\n– Raffaelea subfusca from Craig
\n– Ambrosiella hartigii from Martin Sigut
\n– Fusarium sp. from Martin Sigut
\n– Raffaelea lauricola from Marc
\n– Diplodia from oaks as a free-living control (or some better species?)<\/p>\n

If these fungi are in decently clean cultures, we can subculture them directly into a competitive assay. If they need some cleanup, we will first subculture them alone.<\/p>\n

Competitive assay: We will record single-direction reactions: each field has a record of the reactions of the fungus in the upper row. We also need reactions of each to itself, and a growth rate without a competitor. Can we do two replicates of each assay? <\/p>\n

\n\r\n\r\n
<\/th>\nR sub<\/th>\nR lau<\/th>\nA har<\/th>\nF sp<\/th>\nDipl<\/th>\n<\/tr>\n<\/thead>\n
R sub<\/td>\nsame-growth decrease?<\/td>\nR lau reaction<\/td>\nA har reaction<\/td>\nF sp reaction<\/td>\nDipl reaction<\/td>\n<\/tr>\n\r\n
R lau<\/td>\nR sub reaction<\/td>\nsame-growth decrease?<\/td>\nA har reaction<\/td>\netc<\/td>\netc<\/td>\n<\/tr>\n\r\n
A har<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n\r\n
F sp<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n\r\n
Dipl<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n\r\n
no competitor<\/td>\nR sub growth<\/td>\netc<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody><\/table><\/div>\n

Better ideas welcome!
\nHow should we score it? How about Strong inhibition, medium, weak, no effect? Or estimated percentage of growth decrease compared to the no-competitor control??
\nThank you!<\/p>\n","protected":false},"excerpt":{"rendered":"

Adam, Thank you in advance for your help with Latasha. Here is a summary of what we will do with Latasha on her first visit: If she has beetles, we will identify them, and grind them up to extract symbionts. Here is how. We will plate them and wait a week. If she doesn’t have […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"","footnotes":""},"categories":[17],"tags":[],"class_list":["post-1375","post","type-post","status-publish","format-standard","hentry","category-adam"],"_links":{"self":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1375","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/comments?post=1375"}],"version-history":[{"count":5,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1375\/revisions"}],"predecessor-version":[{"id":1376,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1375\/revisions\/1376"}],"wp:attachment":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/media?parent=1375"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/categories?post=1375"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/tags?post=1375"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}