{"id":1683,"date":"2015-01-28T21:56:39","date_gmt":"2015-01-29T02:56:39","guid":{"rendered":"http:\/\/www.ambrosiasymbiosis.org\/labprotocols\/ampure-magnetic-dna-clean-up\/"},"modified":"2015-10-19T08:35:45","modified_gmt":"2015-10-19T12:35:45","slug":"ampure-magnetic-dna-clean-up","status":"publish","type":"post","link":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/ampure-magnetic-dna-clean-up\/","title":{"rendered":"AmPure magnetic DNA clean up"},"content":{"rendered":"<p>Adam,<br \/>\nWe bought the Ampure DNA purification system. Would you be able to put it together, explore how it works, and give us training?<br \/>\nIt might be worth taking a PCR product that is no longer needed but had a strong band (something that we already sequenced) and runningon a gel 10 5ul of the original, 2) 5ul Ampure cleaned-up version, and 3) 5ul MoBio cleaned version.<br \/>\nThe sooner the better (within two or three weeks?), because I am imagining that Craig and possibly Tyler Dreaden might want to use it for the next MiSeq library.<br \/>\nFor individual Sanger sequencing submission, we will still be using ExoSap. This is only for large volumes that have to be really clean, like whole library submissions.<br \/>\nThank you!<br \/>\nJ<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Adam, We bought the Ampure DNA purification system. Would you be able to put it together, explore how it works, and give us training? It might be worth taking a PCR product that is no longer needed but had a strong band (something that we already sequenced) and runningon a gel 10 5ul of the [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"yes","footnotes":""},"categories":[17],"tags":[],"class_list":["post-1683","post","type-post","status-publish","format-standard","hentry","category-adam"],"_links":{"self":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1683","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/comments?post=1683"}],"version-history":[{"count":1,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1683\/revisions"}],"predecessor-version":[{"id":1806,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1683\/revisions\/1806"}],"wp:attachment":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/media?parent=1683"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/categories?post=1683"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/tags?post=1683"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}