{"id":1744,"date":"2015-05-25T14:10:07","date_gmt":"2015-05-25T18:10:07","guid":{"rendered":"http:\/\/www.ambrosiasymbiosis.org\/labprotocols\/plate-photography\/"},"modified":"2015-10-19T08:35:32","modified_gmt":"2015-10-19T12:35:32","slug":"plate-photography","status":"publish","type":"post","link":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/plate-photography\/","title":{"rendered":"Plate photography"},"content":{"rendered":"

 <\/p>\n

    \n
  1. Download Canon EOS Utility to your computer: Download link<\/a>
    \n(Listed as \u201cEOS Digital Solution Disk Software\u201d)<\/li>\n
  2. Ensure photo setup is as pictured (in the flow hood in Adam’s office on the 2nd floor):
    \n    \"IMG_1341\"<\/a><\/li>\n
  3. Hook your computer up to the camera to enable live shooting and direct photo downloads.<\/li>\n
  4. Turn on the hood blower and wipe down all surfaces with 70% ethanol.<\/li>\n
  5. Set the camera dial to M (Manual) and adjust to the following settings:
    \nManual focus
    \nLarge resolution (use Raw for poster photos)
    \nShutter 1\/100
    \nF9.0
    \nISO 400
    \nSpot metering
    \nFlash white balance setting
    \nWB Shift: B5 G2
    \nAs it appears on the EOS Utility:
    \n\"EOS<\/em><\/li>\n
  6. Set a plate open-side-up underneath the camera and manually focus the camera to the top of the agar.<\/li>\n
  7. Add a piece of paper displaying the underlying plate number in the photo.<\/li>\n
  8. Proceed taking photographs of the top and bottom of each plate. Settings do not need to be altered between plates\/photographs.<\/li>\n
  9. Save photographs to our lab drive (Forest Entomology:\\Plate photos) and name them according to plate number (eg: \u201cplate number XXXX.jpg\u201d)<\/li>\n
  10. \n

    Keep the plate at 4-10\u00b0 C until ready for discard. Plates should be discarded into an autoclave bag.<\/p>\n

    **Note on batteries for flash: Only use Panasonic eneloop batteries. All batteries should be equally charged. Disconnect batteries after use. Battery charger is in camera drawer in Hulcr lab.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

      Download Canon EOS Utility to your computer: Download link (Listed as \u201cEOS Digital Solution Disk Software\u201d) Ensure photo setup is as pictured (in the flow hood in Adam’s office on the 2nd floor): Hook your computer up to the camera to enable live shooting and direct photo downloads. Turn on the hood blower and […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"yes","footnotes":""},"categories":[5],"tags":[],"class_list":["post-1744","post","type-post","status-publish","format-standard","hentry","category-fungi"],"_links":{"self":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1744","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/comments?post=1744"}],"version-history":[{"count":12,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1744\/revisions"}],"predecessor-version":[{"id":1803,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/1744\/revisions\/1803"}],"wp:attachment":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/media?parent=1744"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/categories?post=1744"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/tags?post=1744"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}