{"id":33,"date":"2013-02-07T16:05:09","date_gmt":"2013-02-07T21:05:09","guid":{"rendered":"http:\/\/www.ambrosiasymbiosis.org\/labprotocols\/field-work\/"},"modified":"2015-10-19T08:50:15","modified_gmt":"2015-10-19T12:50:15","slug":"field-work","status":"publish","type":"post","link":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/field-work\/","title":{"rendered":"Field Work"},"content":{"rendered":"<h2>Equipment for field sampling<\/h2>\n<ul>\n<li>autoclaved screw-top vials with moist paper (if sampling live beetles)<\/li>\n<li>autoclaved empty screw-top vials<\/li>\n<li>ethanol<\/li>\n<li>squirt bottle (or separate vials with ethanol)<\/li>\n<li>bullet box for vials<\/li>\n<li>larger tubes<\/li>\n<li>chainsaw + gas + oil<\/li>\n<li>2 broad chisels<\/li>\n<li>crow bar<\/li>\n<li>anvil pruner<\/li>\n<li>folding saw<\/li>\n<li>hammer<\/li>\n<li>hatchet<\/li>\n<li>soft forceps on a string<\/li>\n<li>hard forceps<\/li>\n<li>scalpel<\/li>\n<li>scalpel blades<\/li>\n<li>lighter (for forceps &amp; scalpel sterilization)<\/li>\n<li>ziploc bags<\/li>\n<li>permanent markers (alcohol-resistant)<\/li>\n<li>pigma pen for labels<\/li>\n<li>notebook<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h2>In the field<\/h2>\n<ol>\n<li><b>beetles:<\/b> pierce head, put in numbered vial, record<\/li>\n<li><b>larvae &amp; frass:<\/b> put in numbered vial or tube, record<\/li>\n<li><b>fungi:<\/b> same as above<\/li>\n<li><b>frontalis:<\/b> small samples tied up with rubber band, in ziploc bag<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<h2>Sampling bacteria from beetles<\/h2>\n<p>Up to 3 larval chambers, and up to 3 adult beetles (ideally new generation) per tree<\/p>\n<p>1. surface wash 2. the beetle crushed (elytra removed, surface-washed in bleach, water, ethanol, and dried out)<\/p>\n<p>&nbsp;<\/p>\n<h2>Materials for dissection<\/h2>\n<p><b>(all autoclaved)<\/b><\/p>\n<ul>\n<li>eppendorf vials<\/li>\n<li>pestles<\/li>\n<li>(chisel)<\/li>\n<li>pruner<\/li>\n<li>pipette<\/li>\n<li>1000 uL pipette tips<\/li>\n<li>EtOH bath<\/li>\n<li>scalpel<\/li>\n<li>vial rack<\/li>\n<li>surface wash for adult beetles (elytra removed, surface-washed in bleach, water, ethanol, and dried out)<\/li>\n<li>buffer<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Equipment for field sampling autoclaved screw-top vials with moist paper (if sampling live beetles) autoclaved empty screw-top vials ethanol squirt bottle (or separate vials with ethanol) bullet box for vials larger tubes chainsaw + gas + oil 2 broad chisels crow bar anvil pruner folding saw hammer hatchet soft forceps on a string hard forceps [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_s2mail":"yes","footnotes":""},"categories":[4],"tags":[],"class_list":["post-33","post","type-post","status-publish","format-standard","hentry","category-bacteria"],"_links":{"self":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/33","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/comments?post=33"}],"version-history":[{"count":1,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/33\/revisions"}],"predecessor-version":[{"id":1864,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/posts\/33\/revisions\/1864"}],"wp:attachment":[{"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/media?parent=33"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/categories?post=33"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ambrosiasymbiosis.org\/labprotocols\/wp-json\/wp\/v2\/tags?post=33"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}